Pyridine Nucleosidase in Bull Semen ll. Biochemical Properties
نویسندگان
چکیده
منابع مشابه
Purification and properties of pyridine nucleosidase (glycosidase) from bull semen.
A soluble pyridine nucleotidase was first described in bull semen by Leone and Bonaduce (1). During studies on the specificity of this enzyme, we observed that passage of dilute semen through a column composed of calcium phosphate gel and diethylaminoethyl cellulose anion exchanger (Whatman)] resulted in a preparation of high specific activity. In this single purification step the specific acti...
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A procedure is described for the purification of bull semen NAD nucleosidase. The enzyme was purified over 400-fold to a final specific activity of 9906 units per mg of protein. A spectrophotometric assay was developed in which the enzyme-catalyzed release of 3-acetylpyridine from d-acetylpyridine adenine dinucleotide was followed by measuring the increase in optical density at 232 mp. At pH 7....
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1975
ISSN: 0004-9417
DOI: 10.1071/bi9750273